| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125865 |
| Chromosome: | chromosome 14 |
| Location: | 1937042 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621100 | (1 of 1) IPR000104//IPR001810//IPR025886 - Antifreeze protein, type I // F-box domain // Phloem protein 2-like | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACGACATGGCAGCCCAAAAAGCTGTCG |
| Internal bar code: | ACATGCTGTGAAACGTTGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 99.4318 |
| LEAP-Seq n confirming: | 525 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCTTACGTTGGACTGCAC |
| Suggested primer 2: | TGAATAAAGCTCCATTCGGG |