Insertion junction: LMJ.RY0402.125886_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.125886
Chromosome:chromosome_12
Location:4020281
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g517050 FAP117 Flagellar Associated Protein sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GGACTCCCACGGCGGTATTGTCAGCAGCAC
Internal bar code:ATTCGACGATGAATGTCCATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:569
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCATTAACACCACGGAGC
Suggested primer 2:ACGTGATAGTGATGGGGAGC