| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.125887 |
| Chromosome: | chromosome 14 |
| Location: | 3049021 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628400 | TSA3 | (1 of 3) 6.1.1.7 - Alanine--tRNA ligase / Alanyl-tRNA synthetase; Alanyl-tRNA-synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGACAGTTTACGGATGTCACCTGAAGTTA |
| Internal bar code: | TCAGTGTCTTTGAACGCCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1074 |
| LEAP-Seq percent confirming: | 99.7519 |
| LEAP-Seq n confirming: | 4020 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCATTGTCGCTCAACTCG |
| Suggested primer 2: | CACAGATCGACCGCTACTCA |