Insertion junction: LMJ.RY0402.125888_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.125888
Chromosome:chromosome 17
Location:1080677
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g703850 DC1,ODA3,ODA-DC1,DCC1 Outer Arm Dynein Docking Complex 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAACATCGACAGCGATCCTATTACTTAAT
Internal bar code:CCGCTACAGGGGCGGTTCGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:745
LEAP-Seq percent confirming:96.6584
LEAP-Seq n confirming:1562
LEAP-Seq n nonconfirming:54
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTTATCGGTGGACACTGC
Suggested primer 2:CCAGCTCCTCGTCTATTTGC