Insertion junction: LMJ.RY0402.125891_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.125891
Chromosome:chromosome 10
Location:3475496
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g444850 CAV7 (1 of 2) PF00520//PF08016//PF16905 - Ion transport protein (Ion_trans) // Polycystin cation channel (PKD_channel) // Voltage-dependent L-type calcium channel, IQ-associated (GPHH); Voltage-gated Ca2+ channel, alpha subunit CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGATGAAGCAGCACAGCAGCGCGTTGCC
Internal bar code:GGCTCGTCGCTATCTGAGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:75
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGGATCCAGTATGCAGAGGA
Suggested primer 2:CTGACGTAACCGAGGGTGTT