| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125896 |
| Chromosome: | chromosome 2 |
| Location: | 6713284 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118050 | CYCU1 | U-type cyclin; (1 of 1) PTHR15615:SF20 - CYCLIN FAMILY PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAATGCCGCTGTTTGCAAAGTGGAGCGGC |
| Internal bar code: | CGCGCGCGATATGCCAACCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 98.7654 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACAAAGGTGGGGGAGTA |
| Suggested primer 2: | ACAGAGCATCTACCGGGATG |