Insertion junction: LMJ.RY0402.125904_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.125904
Chromosome:chromosome 7
Location:5081902
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g347600 (1 of 1) PTHR11059:SF0 - DNA REPAIR PROTEIN RECN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCGGCCGCCGCCATGAACGTACATCCA
Internal bar code:CTGTCGCTTGGTTTGGACCCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:860
LEAP-Seq percent confirming:61.082
LEAP-Seq n confirming:1750
LEAP-Seq n nonconfirming:1115
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGGGGGTGAGAATGTGT
Suggested primer 2:AGCGCTGCTACTCCTTCATC