| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125910 |
| Chromosome: | chromosome 1 |
| Location: | 5059821 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035000 | (1 of 1) K14791 - periodic tryptophan protein 1 (PWP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCTAGTGCAGGTACGACGAAACGCTCA |
| Internal bar code: | TCGGGACTTCAGCAGTATCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 99.8435 |
| LEAP-Seq n confirming: | 638 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCTTGTCGCTCTTGTCC |
| Suggested primer 2: | CGAGGCTCAAGAGTCAAACC |