| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125911 |
| Chromosome: | chromosome 2 |
| Location: | 6517330 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116650 | (1 of 7) 3.5.1.52 - Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase / N-oligosaccharide glycopeptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCTACGGTGGCTACGTGAGCGCTAAGC |
| Internal bar code: | AAACGTTCTACATTATTCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 392 |
| LEAP-Seq percent confirming: | 68.0412 |
| LEAP-Seq n confirming: | 132 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGAGCAGTGGTGGAGAG |
| Suggested primer 2: | CTCACCTTGCGGAACTTCTC |