| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.125943 |
| Chromosome: | chromosome 14 |
| Location: | 2611119 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625950 | PAP4 | (1 of 1) 2.7.7.19//2.7.7.72 - Polynucleotide adenylyltransferase / RNA adenylating enzyme // CCA tRNA nucleotidyltransferase / tRNA-nucleotidyltransferase; Class-II RNA nucleotidyl transferase 4, mitochondrial | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAGGGTGGGGGGAGCCGCTAATGACCT |
| Internal bar code: | GGGTTCGAAGGGTGTGACGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 99.8315 |
| LEAP-Seq n confirming: | 1185 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATTCTGATACTGCGGCT |
| Suggested primer 2: | CCAAAGCAAGCCAAACTAGC |