| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125949 |
| Chromosome: | chromosome 6 |
| Location: | 5947530 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g288700 | GYD1 | Glycolate dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAAGGCATCCTGGACACTGGCATTCAGT |
| Internal bar code: | ACCCCAGTAAGAACCCCTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 97.7333 |
| LEAP-Seq n confirming: | 1466 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGACAACAGGCTTATCGG |
| Suggested primer 2: | GTGGCGTGGTCTATAGGCAT |