| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125952 |
| Chromosome: | chromosome_5 |
| Location: | 2244020 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g234662 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCGCTCCATAACCACAGGCAGCTACCCAG |
| Internal bar code: | TTGGTACGGTGCGTGCCAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1008 |
| LEAP-Seq percent confirming: | 98.7337 |
| LEAP-Seq n confirming: | 2885 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCAATTGCCTTTTGTTGC |
| Suggested primer 2: | AGGATGGCATTACCACTTGC |