Insertion junction: LMJ.RY0402.125998_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.125998
Chromosome:chromosome 2
Location:580518
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g077650 (1 of 2) IPR000340//IPR000387//IPR029021 - Dual specificity phosphatase, catalytic domain // Tyrosine specific protein phosphatases domain // Protein-tyrosine phosphatase-like 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCACAACAGCCTCGGCATTCACGCAGAC
Internal bar code:GGTCCCCTCCGAGGGGTCGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:844
LEAP-Seq percent confirming:99.9394
LEAP-Seq n confirming:4949
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:TATACCGACCCCAGCAAGTC
Suggested primer 2:TCGCACTTATGGTACAAGCG