| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.126057 |
| Chromosome: | chromosome 14 |
| Location: | 3712538 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632000 | CGE2,OPR65 | GrpE nucleotide release factor; (1 of 3) K03687 - molecular chaperone GrpE (GRPE) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAGCAGTGCAGCTAACGGCCGGCGGAG |
| Internal bar code: | AGGATGACAGAGGGGGAAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 80.3571 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAGCGCTGCTGCAATAGA |
| Suggested primer 2: | GGACTTTGACAACGCCAAGT |