| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.126113 |
| Chromosome: | chromosome 1 |
| Location: | 1538202 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007950 | CYP2,CYP55B1 | (1 of 1) 1.7.1.14 - Nitric oxide reductase (NAD(P)(+), nitrous oxide-forming) / NOR; Cytochrome P450, CYP55 superfamily, CYP55A family | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATCGGCCCGCTCGGCAGACTTCCCCTTC |
| Internal bar code: | AGGTTAGGTTCGATTAGAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 99.5402 |
| LEAP-Seq n confirming: | 433 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCACGCACTATACCCCTGC |
| Suggested primer 2: | CTGAAGGAGATTCTGGGCTG |