| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.126118 |
| Chromosome: | chromosome 10 |
| Location: | 2684355 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437900 | SPL31 | (1 of 1) PTHR17204//PTHR17204:SF29 - PRE-MRNA PROCESSING PROTEIN PRP39-RELATED // SUBFAMILY NOT NAMED; Pre-mRNA splicing factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGCCAGCTCGCAGTCAAGTCCTCAGGA |
| Internal bar code: | GCGGAGTTTGAGTCACTCTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1042 |
| LEAP-Seq percent confirming: | 99.1903 |
| LEAP-Seq n confirming: | 245 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACCACTGCACACTGAGCC |
| Suggested primer 2: | GGGTTATGAGGAACAAGGCA |