Insertion junction: LMJ.RY0402.126142_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.126142
Chromosome:chromosome 17
Location:6126296
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g741500 KIL7 Putative kinesin-like motor protein; (1 of 1) 3.4.21.72//3.6.4.4 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease // Plus-end-directed kinesin ATPase / Kinesin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAGCCAGTGCAGGGCGGGCGGCCTGTTG
Internal bar code:CTTTCGACCGGGGTCCGACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:470
LEAP-Seq percent confirming:98.8176
LEAP-Seq n confirming:3343
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGGCTGACAAGCTGTT
Suggested primer 2:CTTGAGCTGCTTCTCCTGCT