| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.126146 |
| Chromosome: | scaffold 24 |
| Location: | 88489 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755747 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGGCCGCCGCCCGCCCGCACAGTTCAC |
| Internal bar code: | ATGTAGTTAGAAGAATAACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 99.8333 |
| LEAP-Seq n confirming: | 599 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTATGCCCCACAACTTCA |
| Suggested primer 2: | TACCGACTGCTGTCATGCTC |