| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.126172 |
| Chromosome: | chromosome 16 |
| Location: | 873315 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648100 | (1 of 1) K05757 - actin related protein 2/3 complex, subunit 1A/1B (ARPC1A_B) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGACGGCCAAGTGCTGCTGTGGGACCTG |
| Internal bar code: | ATCGGCAGCGAGTTTGGCAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 264 |
| LEAP-Seq percent confirming: | 99.6583 |
| LEAP-Seq n confirming: | 9041 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCCATACCTTGAAACACT |
| Suggested primer 2: | TGGCATGCTTCCACTTGATA |