| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.126177 |
| Chromosome: | chromosome 2 |
| Location: | 4793639 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102776 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCCTGCACGAGCAGCAGCAGCAGATAC |
| Internal bar code: | GCGAGGCACCGAAGACGAGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 53 |
| LEAP-Seq percent confirming: | 99.4726 |
| LEAP-Seq n confirming: | 943 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTAGCTATCTGCTCACG |
| Suggested primer 2: | CCATAGCCGTCTAAAGCAGC |