| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.126202 |
| Chromosome: | chromosome 3 |
| Location: | 4752195 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178100 | MOT51 | (1 of 1) PTHR10877//PTHR10877:SF116 - POLYCYSTIN-RELATED // SUBFAMILY NOT NAMED; Predicted protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGAAGCTGACCATGTTCGGCAAGCTGGA |
| Internal bar code: | CGCACGCCGCTTGCCGTCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 99.8323 |
| LEAP-Seq n confirming: | 2976 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTCCTGACATTCCTGGT |
| Suggested primer 2: | CAGCTCGATGCTGATGTTGT |