| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.126219 |
| Chromosome: | chromosome 13 |
| Location: | 2800015 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582950 | 5'UTR/5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAGTCGAAGGTGGGGTGCAGAGCGTGG |
| Internal bar code: | CTTTTCGCTTACGCTTTACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 371 |
| LEAP-Seq percent confirming: | 99.0764 |
| LEAP-Seq n confirming: | 1609 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAACAGGGACTGGAGTTGA |
| Suggested primer 2: | TGCTTGCATACGAAACGAAG |