| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.126229 |
| Chromosome: | chromosome 3 |
| Location: | 5193678 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182550 | PNO3 | FAD-dependent pyridine nucleotide-disulphide oxidoreductase; (1 of 1) 1.18.1.3 - Ferredoxin--NAD(+) reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCGCCCGCAAGCTTTACCACCTGAGGA |
| Internal bar code: | ATGAGATAATGTAGCAACTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 99.433 |
| LEAP-Seq n confirming: | 1403 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCAGCACATCTGACTTTC |
| Suggested primer 2: | GTAGTACCAAGGCTGGCTGC |