Insertion junction: LMJ.RY0402.126248_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.126248
Chromosome:chromosome 11
Location:2890431
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g477850 TXC1,TSN1 (1 of 1) K15979 - staphylococcal nuclease domain-containing protein 1 (SND1); Transcriptional coactivator-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGATATTTTTTGGTGCATCATTCAGCCG
Internal bar code:TGCAGCTCCGTTCAGGATGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:534
LEAP-Seq percent confirming:99.7285
LEAP-Seq n confirming:2571
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GTTAGTTGGGGTTGGAGGGT
Suggested primer 2:ACACGCACGTCTGAATGAAG