Insertion junction: LMJ.RY0402.126256_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.126256
Chromosome:chromosome 7
Location:2177009
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g326800 PPP29,PP5,CCPP5 Co-chaperone protein p5 of HSP90; (1 of 1) PTHR11668:SF21 - SERINE/THREONINE-PROTEIN PHOSPHATASE 5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAATCCTGAATCCACCTTGAACCGCCCCC
Internal bar code:TTCTCACGTGGACTTTTCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:106
LEAP-Seq percent confirming:98.7179
LEAP-Seq n confirming:154
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGTAGTAGCCCTGTGTGT
Suggested primer 2:CTTTATGCCGGCACTTGTTT