Insertion junction: LMJ.RY0402.126260_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.126260
Chromosome:chromosome 7
Location:5420539
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g350400 PIN3 Peptidyl-prolyl cis-trans isomerase, parvulin-type; (1 of 1) 2.7.7.80//2.8.1.11//5.2.1.8 - Molybdopterin-synthase adenylyltransferase // Molybdopterin-synthase sulfurtransferase / Molybdopterin synthase sulfurylase // Peptidylprolyl isomerase / Rotamase CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTGTGTGGAGGGGAAGGTTGGCAACTCA
Internal bar code:CTGCCGTTTCGAACCCTTAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:687
LEAP-Seq percent confirming:95.6621
LEAP-Seq n confirming:1257
LEAP-Seq n nonconfirming:57
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ATACCCATGTGACGAACGGT
Suggested primer 2:TCGCCTGAGTCAGCACATAC