| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.126304 |
| Chromosome: | chromosome 16 |
| Location: | 5765191 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678551 | CYG34 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTCCAACTTGCCATGCTTGCCATGCCGA |
| Internal bar code: | ATTCAGGGCATTGATTGCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 99.5375 |
| LEAP-Seq n confirming: | 1937 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATGATTACAGCGCCCTCC |
| Suggested primer 2: | TTCGGTGTGTACAAGGTGGA |