Insertion junction: LMJ.RY0402.126313_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.126313
Chromosome:scaffold 36
Location:24006
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre36.g759747 (1 of 26) IPR029058//IPR029059 - Alpha/Beta hydrolase fold // Alpha/beta hydrolase fold-5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAAGAAGCGAAGGGTTGGGGTAAGTGGGG
Internal bar code:GGATTCGCCATCAGTCGGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:143
LEAP-Seq percent confirming:95.4074
LEAP-Seq n confirming:1932
LEAP-Seq n nonconfirming:93
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCCTCAAGACGTAGGCAC
Suggested primer 2:TTTCTCGACTTCCTCGCAAT