| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.126324 |
| Chromosome: | chromosome 15 |
| Location: | 5214 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g634500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGAGCCCCACGAGCGCGCTGCAGCAGA |
| Internal bar code: | CCCTGTCGTGTAAGGGGTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 13 |
| LEAP-Seq percent confirming: | 70.2128 |
| LEAP-Seq n confirming: | 132 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTCACTCCTGACACCAC |
| Suggested primer 2: | CAGGAAGCACACAGGAGTCA |