| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.126333 |
| Chromosome: | chromosome 9 |
| Location: | 5729717 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401738 | (1 of 1) K17619 - magnesium-dependent phosphatase 1 [EC:3.1.3.48 3.1.3.-] (MDP1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCTGAACGGCTAATTGTTGCTCTGCCC |
| Internal bar code: | TTAATTATCTCAGCTGTTAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 86.2564 |
| LEAP-Seq n confirming: | 841 |
| LEAP-Seq n nonconfirming: | 134 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGATTGTGAGTGGGGGT |
| Suggested primer 2: | GTGTAGACGCACACAATGCC |