| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.126348 |
| Chromosome: | chromosome 7 |
| Location: | 3266451 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334300 | TRP6 | Transient receptor potential ion channel protein; (1 of 2) PTHR22847//PTHR22847:SF442 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTCACGGGTAACAAAGCATGCCGGGACG |
| Internal bar code: | GGATTTTACACCTATCCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 887 |
| LEAP-Seq percent confirming: | 99.0868 |
| LEAP-Seq n confirming: | 651 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGGAACACCGAGACAGG |
| Suggested primer 2: | CTTGTGTTGTTGCTGGCACT |