Insertion junction: LMJ.RY0402.126371_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.126371
Chromosome:chromosome 2
Location:4012216
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g097050 (1 of 8) PF01697 - Glycosyltransferase family 92 (Glyco_transf_92) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCAACCCGTCACACTCTTGTCGACTGAT
Internal bar code:TGTCACGGCCGCACCGGGTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:870
LEAP-Seq percent confirming:99.2531
LEAP-Seq n confirming:2392
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGTAAGACAGGGACCCCC
Suggested primer 2:GATTGTTTGTGGCTGGTCCT