| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.126390 |
| Chromosome: | chromosome 4 |
| Location: | 508815 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217934 | (1 of 1) K07101 - uncharacterized protein (K07101) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCAACCCACGCAAGTCCGCACCCCACG |
| Internal bar code: | TGGGTTGCTTTGCGGCGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 511 |
| LEAP-Seq percent confirming: | 99.6441 |
| LEAP-Seq n confirming: | 1120 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTAAGGCCCTTGCACTT |
| Suggested primer 2: | CGGATGAGCTGTGTAGACGA |