Insertion junction: LMJ.RY0402.126431_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.126431
Chromosome:chromosome 3
Location:7668083
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g203950 (1 of 3) 1.11.1.10 - Chloride peroxidase / Vanadium haloperoxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCAATGACCGCCACACGCCGCAGCCCCA
Internal bar code:CGAGTACTGGCATTGATCCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:389
LEAP-Seq percent confirming:99.5735
LEAP-Seq n confirming:2568
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GATTGCAGCTGACCACAAGA
Suggested primer 2:ATGGAAACCGTAGGGAAAGG