Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.126443 |
Chromosome: | chromosome_10 |
Location: | 5449347 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g458600 | FAP245 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCGCTAGGGACTGCCGGACCGCCTCCGCGC |
Internal bar code: | CACCGAGAGGTAGGGTGCTTAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 922 |
LEAP-Seq percent confirming: | 91.898 |
LEAP-Seq n confirming: | 1622 |
LEAP-Seq n nonconfirming: | 143 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGTTCAGTCCATCAAGCCC |
Suggested primer 2: | GAGGTGAGTTGGCGCTTTAG |