| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.126486 |
| Chromosome: | chromosome_3 |
| Location: | 2567590 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| None |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTGAAAATTTGCTTTCGTACGACAGAAAC |
| Internal bar code: | GAGGGATATAGGGGGGGTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 99.7574 |
| LEAP-Seq n confirming: | 2056 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGTTACCGCCCACAAAT |
| Suggested primer 2: | ATGTCTGGCGACATAGAGGG |