| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.126486 |
| Chromosome: | chromosome_13 |
| Location: | 1908428 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g576000 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCAGTCGCCGGGCAAACAGGGCCGAGCCC |
| Internal bar code: | GTGTATTGCCAGCTCGGCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 891 |
| LEAP-Seq percent confirming: | 95.4458 |
| LEAP-Seq n confirming: | 2976 |
| LEAP-Seq n nonconfirming: | 142 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGGTGCCTGTGACTACA |
| Suggested primer 2: | GTTGGTCACGGTAGCCATCT |