| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.126492 |
| Chromosome: | chromosome 6 |
| Location: | 7060051 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296983 | SAE2 | (1 of 1) K10685 - ubiquitin-like 1-activating enzyme E1 B (UBLE1B, SAE2, UBA2); SUMO activating enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGTATAGCGACGCATCGAGAACCCTTT |
| Internal bar code: | TATGGAATTTCGTACTCCTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 252 |
| LEAP-Seq percent confirming: | 93.0314 |
| LEAP-Seq n confirming: | 267 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCAACATCATCTCGTCTT |
| Suggested primer 2: | ACATCCTCATAGCAGCCCAC |