Insertion junction: LMJ.RY0402.126494_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.126494
Chromosome:chromosome 6
Location:310152
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g251250 HIK3,HKR3,HIK (1 of 1) PF00072//PF00512//PF02518 - Response regulator receiver domain (Response_reg) // His Kinase A (phospho-acceptor) domain (HisKA) // Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c); Histidine kinase response regulator of two-component system 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCTGCAACGCTTCAAGGCGTACACTCTT
Internal bar code:CGGATTGGTCGCGATTGCATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:131
LEAP-Seq percent confirming:99.2857
LEAP-Seq n confirming:278
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GATGAGGCACAACTGGGTTT
Suggested primer 2:CCCATCAAGCTGTAGGCAGT