| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.126497 |
| Chromosome: | chromosome 5 |
| Location: | 2255917 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234664 | (1 of 2) 4.2.1.93 - ATP-dependent NAD(P)H-hydrate dehydratase / ATP-dependent H(4)NAD(P)OH dehydratase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACATGCTCAGGGACCACATGCTCTGGT |
| Internal bar code: | AGGCGTCCCAACTGAATGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 155 |
| LEAP-Seq percent confirming: | 99.6552 |
| LEAP-Seq n confirming: | 867 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATGTGTGGCGTTGATTAG |
| Suggested primer 2: | CAAAGCCATTGTTTCCTCGT |