Insertion junction: LMJ.RY0402.126499_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.126499
Chromosome:chromosome 9
Location:2069674
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g394050 CPLD19 Zinc finger family protein; (1 of 1) PTHR22883:SF91 - PROTEIN S-ACYLTRANSFERASE 12-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTAGTTTGGGCTGGTGCTTACAACCCCG
Internal bar code:GGATTGCCTCTCAATGGGACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:89
LEAP-Seq percent confirming:99.435
LEAP-Seq n confirming:704
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ATACGTCCTCCCAAACCTCC
Suggested primer 2:TGTCTGTGTCTCCAACGCTC