| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.126531 |
| Chromosome: | chromosome 5 |
| Location: | 2466247 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235500 | KIN7A,KIN7-1 | Kinesin motor protein; (1 of 1) PTHR24115//PTHR24115:SF70 - FAMILY NOT NAMED // KINESIN-RELATED PROTEIN 11 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTAGCTGCCTGCTGACCAGCGATGGGG |
| Internal bar code: | TCGGGCTATGACGCTGCGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 99.8126 |
| LEAP-Seq n confirming: | 2663 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTAGATTCCAGCCCAAA |
| Suggested primer 2: | CACGAGCTGAGACAGCGTAG |