| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.126544 |
| Chromosome: | chromosome 7 |
| Location: | 2252869 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327500 | (1 of 3) PF10358 - N-terminal C2 in EEIG1 and EHBP1 proteins (NT-C2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGGCCGCCCCTCGCCCTGCGTGCACAT |
| Internal bar code: | GCAACGCTGGAGCCTCGTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 985 |
| LEAP-Seq percent confirming: | 99.8334 |
| LEAP-Seq n confirming: | 8990 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGTAGTCACCGCTCCTT |
| Suggested primer 2: | GAGAGCATAAGGTTCGACGC |