| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.126563 |
| Chromosome: | chromosome 10 |
| Location: | 2569793 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436850 | (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCGAACGCGCAGGTGGCTCGAGGCTCT |
| Internal bar code: | TACTTGGTTTGTGGGCCGGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 97.1698 |
| LEAP-Seq n confirming: | 1339 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTACCTGCGTATGTGGGGT |
| Suggested primer 2: | TGATAGGCAGTAAAGGCGCT |