| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.126563 |
| Chromosome: | chromosome 16 |
| Location: | 5898878 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677200 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTCGTGGCTCTAGAACACCAGCACGCC |
| Internal bar code: | CAGAGTTCCGGATCGTACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 99.7616 |
| LEAP-Seq n confirming: | 2092 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTTGTCGACTTTCGCTT |
| Suggested primer 2: | TGAAACGTATGCTGAGTCGC |