Insertion junction: LMJ.RY0402.126600_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.126600
Chromosome:chromosome_6
Location:2184181
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre06.g265950 DHC3 Flagellar inner arm dynein heavy chain, novel minor type dynein sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TCCCATCATCATCAGAAAGGTGTAAGTCAC
Internal bar code:GAGCGGGGAAAGGTGCGGACGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:865
LEAP-Seq percent confirming:99.8195
LEAP-Seq n confirming:1106
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTCGCTGATCTCCACAGA
Suggested primer 2:GCTTCTTCACCACTGCATGA