| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.126633 |
| Chromosome: | chromosome 7 |
| Location: | 4675500 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344668 | (1 of 2) IPR000104//IPR004827 - Antifreeze protein, type I // Basic-leucine zipper domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACACATGGCTGCGCCTAATGGACGCTT |
| Internal bar code: | GTCAGGAACCCCCCGGCTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 820 |
| LEAP-Seq percent confirming: | 99.8117 |
| LEAP-Seq n confirming: | 6361 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCCTGTGGGTGTCTGGT |
| Suggested primer 2: | CTCAGCTACTGCGTGCTGTC |