| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.126635 |
| Chromosome: | chromosome 7 |
| Location: | 3279224 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334350 | (1 of 3) 2.7.1.39 - Homoserine kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCTCAACACGCTGTTTGACGTGCCCTCA |
| Internal bar code: | CAGGGGCCGGTAAGTCAGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 819 |
| LEAP-Seq percent confirming: | 60.2059 |
| LEAP-Seq n confirming: | 2690 |
| LEAP-Seq n nonconfirming: | 1778 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCGTCTGAACTTGCTGG |
| Suggested primer 2: | TGCGGTGTTTGCTTTAAGTG |