| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.126637 |
| Chromosome: | chromosome 6 |
| Location: | 1648731 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260950 | (1 of 1) K12483 - EH domain-containing protein 1 (EHD1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGCCCACTAGCTCGCTAGCTCGCTATCA |
| Internal bar code: | TAGGACGACGGTCCTACGAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 99.6054 |
| LEAP-Seq n confirming: | 5806 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCTTTCTGCGGTGGACT |
| Suggested primer 2: | TTCTAGCGGCAAGGGTAATG |