| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.126653 |
| Chromosome: | chromosome 2 |
| Location: | 5893948 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g111500 | (1 of 1) PTHR24056//PTHR24056:SF7 - CELL DIVISION PROTEIN KINASE // CYCLIN-DEPENDENT KINASE-LIKE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCATCAGGCGGGCGGTGCGCTTGGCTCG |
| Internal bar code: | TGTGCGGTCCTAGCACTTCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 99.5595 |
| LEAP-Seq n confirming: | 226 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAGCACACGCACAGAAG |
| Suggested primer 2: | ATATTGGTGCCAGACCAAGC |